Coding

Part:BBa_K5035002:Design

Designed by: Monica Barsever   Group: iGEM24_IEA   (2024-09-11)


T7RdhA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E.coli N terminal His tag added


Source

Reference paper

References